5 Gel Electrophoresis of Proteins
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Immunogens of Brucella Abortus S19 Identified By Two-Dimensional Gel Electrophoresis and Immunoblotting
Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction b...
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Two–Dimensional Gel Electrophoresis of Ceolomic Fluid of Eisenia foetida Earthworm
Earthworms possess antioxidant, antibacterial, antitumor, and hemolytic properties. To recognize the molecules responsible for various biological activities of earthworm’s coelomic fluid, a detailed knowledge about its protein contents is required. The aim of this study was to characterize the proteins present within the coelomic fluid of Eisenia foetida earthworm. Polyacrylamide-gel-elec...
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Seed proteins of eight species of the genus Suaeda (Chenopodiaceae) were compared by means of polyacrylamide disc gel electrophoresis. Different samples of the same species showed not less than 89% similarities, and geographical separation had no remarkable effect on seed protein patterns of the same species. Morphological heterogenity, that creates difficulties in infrageneric classificat...
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In order to examine radiation-induced proteins in an extremely radio-resistant bacterium, it became possibleto perform comparative proteomic analysis on radio-resistance Bacillus megaterium WHO as a wildtypestrain for the first time. Variation in cellular proteins profiles of the Bacillus megaterium WHO after 5KGy γ-irradiation were analyzed by two-dimensional poly acryl amide...
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تاریخ انتشار 2017